Indoor living soil- the knowledge base

Honorary mentions and introduction to the Living Soil Community

I hereby try to compile as good as possible my knowledge collected so far. It is tried to work with the current scientific knowledge, which is constantly changing. Therefore, some information may already be refuted again. Many techniques have also not yet been explicitly determined for cannabis and there is also not much research on multi-cyclic soil systems based on peat. That's why I'm trying to combine anecdotal and scientific knowledge to give the most comprehensive and effective guide to gardening with Living soil for cannabis.

I am already looking forward to lively discussions and also to learn from you. I only ask for a scientific style of argumentation, i.e. just please work with sources and if there are none, argue with data and own experiences.

You can reach me via the Instagram accounts Bavarian_buds/Research-gardens or via the comment function

This article is based on my studies in horticultural science, William Albrecht's soil fertility principle studies, Tad Hussey's podcast "Cannabis Science and Cultivation", Bryant Mason's advice on my soil samples/values, and many people from the forums such as Jonathan Traxler or Nino's Farm from the Indoor Living soil community whose recipes and tips are used as the basis for the sample soil mix included in the third part of the article series.

The plan for this series of articles is to give the basics needed to assemble and grow a "Living Soil" system on your own in three parts.
The first part will be about the general concept, sustainability aspects and the basics of a "good" soil for cannabis. It will break down the three dimensions in which good soil is evaluated (Physical/Chemical/Biological) and highlight the key influencing factors. It can also be considered as a basic knowledge section.

The second part will revolve around the translation of the previously listed quality characteristics into the soil composition of the basic substances, such as peat, via minerals and composts. It follows both an explanation of the test methods and quality characteristics of the individual substances and the soil mixed together to determine from it in process control values to standardize and optimize production as much as possible.

In the third and last part we will go into the culture management of the soil, because this is at least as crucial to success as the right composition of the mix. Finally, I will attach a list and an Excel calculator of my preferred mix for the people who want to start directly with a well thought-out mix.

The concept of Living Soil and the term Modified Growing Mix

Definition

Indoor living soil is currently experiencing a real hype, which is also accompanied by the spread of some misinformation that requires correction. A real living soil, as it was originally called in the permaculture movement in cannabis, describes an outdoor soil in a specific microclimate with local influences of wind, weather and native soil, which is enriched with organic and mineral nutrients such as compost or rock dust, adapted to the plant needs. It has a complete ecosystem that we can only slightly influence, but which, with the right conditions, can have a very positive effect on cultivation. For example, an optimal balance of the system can withstand strong pest pressure through increased bird nesting.

Over time, a biotope of its own develops here and, with proper care, a balance is established. This regulates most problems by itself, such as nutrient deficiencies or drastic pest infestations. We have to create this balance in the indoor system from scratch and can only partially fall back on established biotopes like outside.

Furthermore, we work here with native soil made of sand, silt, clay and rock. This is difficult to implement indoors or has not been experimented with much. The anecdotal evidence I have received so far as to why a peat-based system works better is limited to the high aggregation stability of peat over time, a good pore ratio, a high cation exchange capacity, and the low PH of peat, which allows for high accumulation of cations such as calcium/magnesium. It follows that we don't actually use "soil" at all for "indoor living soil", but a "soilless" mix, as it is known in the English-speaking world. By adding rock meals and other nutrient sources we modify this "soilless" mix even further so the name is changed to modified (soiless) growing mix (MGM) and this is also used as the correct name in the rest of the article.

Indoor living soil is usually referred to a bed or a potting system with at least 60-80L of substrate, which is peat-based and purified by means of minerals / organic fertilizers and used over several runs. Here the goal is to create a diverse and active community of microorganisms, including bacteria, fungi and other beneficial insects. These microorganisms play an important role in plant health and growth by providing essential nutrients (Tegeder and Rentsch 2010)improving soil structure (Rillig and Mummey 2006; Helliwell et al. 2014) and plant resistance to harmful organisms (pathogens).

This means that while it makes sense in some aspects to imitate nature, as in the pest example above, it is also sometimes advisable to use new methods, such as the use of peat. Our goal is to increase yield/quality over inputs. I.e. our goal when introducing new techniques is always to either increase output or minimize the amount of input/environmental damage needed to maximize the total break output/input. This should always be the top priority in the evaluation process.

Why the system can also be sustainable indoors

Some people will now wonder if you do not bring in a lot of pests with all the organic matter. This question is justified and, unfortunately, this is true.

However, this pathogen pressure is easily possible with a well-integrated pathogen management system. This includes prevention through purity, useful insects and in case of need organic sprays, such as Neem oil. However, pest management will be a separate article, otherwise it is beyond the scope.

The benefits, on the other hand, are manifold. With proper, local and conscious purchasing of organic fertilizers, living soil can be cheaper and more sustainable per run. However, great care must be taken in what and where one buys, as often fertilizer materials have long shipping distances or unethical predatory methods as with certain guano manufacturers or rock meals that are mined using open pit mining methods. This must be avoided and always buy as seasonally and locally as possible, otherwise the idea of sustainability is quickly undermined. Optimally, an LCA (life cycle assessment) review for the entire mix has been done beforehand, this gives an overview of all externalities (impacts outside the immediate environment on the entire ecosystem) of the components. The MGM shown later will be CO2/externalities optimized, but not perfect, it will be continuously improved. The best option is to integrate as much as possible into your own cycle. I.e. make your own compost, source aggregate meals from local quarries, and enrich ferments yourself.

Now we talk about sustainability, but use peat as a base, why? There are sustainable extraction practices that improve and balance the CO2 balance, which is negatively affected by the outflowing methane during peat extraction. Care must be taken to ensure that the operation offers a regeneration strategy, as simply balancing CO2 does not do much for the locally degraded ecosystem.

An example of this is the Veriflora® certification. It has good properties such as low storage density (see chapter 2.1.1) to balance humus, has good CEC values (chapter 2.2.2), and is stable over many runs (Burgel et al. 2020). This is especially important if one does not want to remix the soil every 3rd time because the substrate slumps. However, peat cannot provide the pure base substance. Which additives are needed and which values are used for evaluation will be summarized in the following chapters.

Why Living Soil can produce superior plant quality and expression

The topic of quality and its definition fills a separate article, which will appear after this series of articles, and therefore only a small excerpt is shown here

The system is divided into three subject areas. The first category describes the pharmaceutical quality. The remaining evaluation criteria are split into the basic craftsmanship of production/processing and the sensory profile. These are to be evaluated in a pyramid-like manner in the sequence of the main categories (1.1; 1.2; 1.3). That means for an optimal product these must be fulfilled build on each other.

It could be compared to Herzberg's two-factor theory, where pharmaceutical quality can be assigned to the hygiene factors and the other two to the motivational factors. (Becker 2019). This means that the material must always at least correspond to the pharmaceutical qualities before one can even think of evaluating taste or flower processing. In the weighting, a distinction must be made according to the market positioning/stakeholdership of the target demographic up to which category level one must go.

The cornerstones of the pharmaceutical definition must be fulfilled for the use of flowers. Especially in the medical field, compliance with pharmaceutical production standards is a basic requirement. These must be adhered to throughout the entire value chain, from cultivation to packaging.

From GACP to EU-cGxP to HACCP guidelines and the more advanced certifications, there are many ways to meet and exceed the specifications of the European Central Laboratory. These refer to the basic test criteria according to the German Pharmacopoeia (DAB) which consist of purity, identity and active ingredient content. Colloquially, this means: Is the flower free of pesticides, heavy metals, and microbial contamination; is it really cannabis; and does it contain as much active ingredient as stated. (Deutscher Apotheker Verlag 2021).

However, it remains to be seen whether GACP/GMP is absolutely necessary for compliance with these basic qualities; my colleague Lorenz has already dealt with this topic in greater depth. All that can be said is that it is possible to create high-quality, pure products even without a 100-million-euro plant.

This quality level is easy to cover with Living soil, you just have to respect the microbial consortium (chapter 2.3) and work with pharmaceutical purity, so no sprays in flower, work only with beard/hairnets/gloves and submit flower tests (COAs - Certificates of analysis).

The second quality level is the craftsmanship quality. Artisanal qualities include factors that can be objectively assessed in end products and are included in the manufacturing process. These include

• The cultivar (ancestral tree and goodness of selection)
• The cultivation (expression of the cultivar, sustainability)
• The drying
• Trim, treatment and storage of the material
• Consumer-owned ratings such as "White ash".

In particular, the cultivation is of course the decisive factor here. And hereunder the expression of the cultivar, which is the expression of the genetic base material under the influence of the grower and the environment arises. So to speak, a miniterroir, when someone deals with wine. It does not describe that one personally likes the olfactory profile, but that the cut has been well germinated.

Haze, for example: many people hate Haze, but a well-growed Haze, when allowed to go its full 15 weeks and possess fine, deep notes, remains a good Haze in terms of craftsmanship. So it puts its own special characteristics in the foreground. Genetics and the quality of its selection obviously comes as a limiting factor here, as a specific cultivar can only be as well grown as it is genetically capable of being. Therefore, at the beginning of every superior product there is an intensive selection, in order to then tease out the best possible expression (dial in process).

This is possible with all cultivation methods, but faster and easier with Living Soil, because it allows the plant itself to decide which nutrients to take up and when to stop, e.g. to achieve optimal maturation/senescence, more details in chapter 2.2.3 The uptake of nutrients at the roots. To achieve this, of course, the hand tool must sit, so the evaluation of these criteria also falls under the craftsmanship quality.

The final level of quality is then rather subjective and often dependent on the testers in its evaluation as it assesses the sensory quality. While this can also be divided into measurements such as terpene concentration, diversity or THC measurements, in my opinion this does not do justice to the delicate differences of the flower and is based on the approach of science to break everything down. I am more than many a vehement advocate of science, but when it comes to stimulants like wine, cigars, spirits, or even cannabis, I still see man up front. That's why I would prefer a rating system like the Robert Parker points for wine for sensory and overall quality over the measurement-based current systems.

Skilled assessors train for years for these jobs, so it is not so easy to get this skill. Especially in the grey area of cannabis, where intensive black market activity is still needed. This is one of our advantages as non-legal market participants over the new people that are now sneaking in all at once once it becomes legal. So hopefully there will always be a place for people with a sense of quality.

A living, organic system can again be an advantage here, as the decomposition processes in the soil produce hundreds of decomposition products such as caproic acid or carbon-based nutrients, which give the plant completely different possibilities to save energy and form additional defense substances, which we are specifically behind in cannabis. In fact, there are also substances like lactones, which are present in tiny amounts, which are not broken down in most tests and yet have a significant impact on the odor profile. This can be seen quite clearly when comparing cannabis based and synthetic based terpene blends for cartridges for example. This value is given in n-butanol reference values.

Figure 1: Odor intensity classification scale compared to n-butanol. (St. Croix Sensory Inc. 2018)

In summary, Living soil makes it easier to create an optimal expression of the cultivar and to reach the maximum genetic potential. Thus, it facilitates quality levels 2/3, but the first level (pharmaceutical quality) is somewhat more difficult to achieve than with an inert rockwool system, as these are much easier to keep pure.

An exact listing and integrative rating system of the current quality definition follows in the article: What constitutes quality in cannabis.

What is the composition of a good soil

But what all do I have to consider when compiling? First of all, we have to look at the "optimal" MGM. This one does not exist, because every situation, every setup and every growstyle is different. That's why you have to put together the tools and adjusting screws and then adapt the soil mix depending on the situation.

For example, if one has a grow with maximum irradiation (1200umol/s*m2) but no watering system with sensor, a soil with high field capacity (water holding capacity) is advantageous. If one wants more short-term control and can re-fertilize often, the use of quickly available fertilizers in lower concentrations is more advantageous than using a large, slowly available amount of organic fertilizer once.

But such strategies come later, for that first the basics must sit. For this purpose, we will first clarify the most important terms on the subject, which are important in indoor living soil. This will by no means be exhaustive, especially from a scientific point of view. I recommend for this the book (Blume et al. 2010) for a full overview of soil science.

Physical factors

Storage density, grain size and aggregation

Storage density is a measure of the mass of soil in a given volume. It is usually expressed in grams per cubic centimeter (g/cm^3) or pounds per cubic foot (lb/ft^3). Soil storage density is an important physical property that can affect the soil's ability to support plant growth and the movement of water and nutrients through the soil.

There are several factors that can affect the storage density of the soil, including the type of soil, the type and amount of organic matter present, and the composition of the pore profile. Soils with a high percentage of organic matter tend to have a lower storage density because the organic matter takes up space and reduces the density of the soil, this is true for proper soils with clay, in our peat based system the density is pretty much the same as the parent material peat. Similarly, soils with a high percentage of pores tend to have a lower storage density because the pores form voids that are not filled with soil particles.

Storage density can also be influenced by soil management practices. For example, tilling the soil with heavy machinery or rough digging, which breaks up the natural aggregrates, can increase storage density.

Understanding soil storage density can be useful for a number of purposes. In agriculture, for example, knowledge of soil storage density can help farmers optimize irrigation and fertilization practices. It can also be used to predict the movement of water and solutes through the soil, which can be important for predicting the risk of soil erosion and leaching of contaminants. But to read out implications for cultivation, we need more data such as grain size, pore composition, aggregation and the target values.

Grains are defined as the composition of the mineral bodies from primary/secondary minerals, e.g. pumice or silicates in the soil. Aggregates are when these grains or organic particles form a complex together, as in peat. If you have ever opened garden soil, you know what I mean, that is not perfectly homogeneous powder, but should have a nice crumb structure. These "pieces" in the soil are compositions of organic matter, microbiome (bacterial slime), minerals and pore distribution.

Figure 2: Black peat, the substrate with almost perfect structure. (International Peatland Society 2019)

In cannabis cultivation specifically, researchers are in the process of determining what the optimal values for storage density / composition are, but in cultivation itself there is unfortunately little data so far. These are often difficult to determine for living soil substrate mixtures, as both the mixture ratios and the microbial composition are inhomogeneous.

Based on my own tests and advice from Jonathan Traxler, I would recommend a density of 0.4-5g/cm³ or 400-500kg/m³ , but again this is dependent on the objective, we took this value to shift the pore balance towards the center pores to maximize contents in oxygen and water holding capacity while making the soil heavy enough to create an appropriately high Total Exchange Capacity.
These concepts and quality characteristics will be discussed in the next chapter and in sec. 2.2.4 CEC and AEC - Cation and Anion Exchange Capacity and Base Saturation will be explained.

For simple home use, a visual assessment of the structure/aggregation is sufficient by taking a handful, moistening it to saturation and squeezing. It should drip slightly and form a "loose" clump, but this will move apart after a short time. You can also lightly dig up the soil itself by hand and see how big the soil aggregates are. There should be just under 3-4cm³ aggregates/clumps, this is also called crumb structure.

There are studies such as. (Campbell et al. 2021)which look at substrate influence using mineral nutrients and hormones on rooting of cuttings. Here there is a pure series of experiments on the influence of substrates on root rate and root weight. All parameters were positively influenced by low storage density and higher porosity up to a certain point. Too high porosity in the sense that there is a lack of contact between roots and soil leads to loss of turgor, the internal water pressure that keeps the plant upright. (LOACH 1985)

Pore size and distribution

Pores are the spaces between the grains and result from their size/storage, which we considered in the last chapter. They play a crucial role in the physical and chemical properties of the soil, as they can affect their ability to absorb water, air and other substances.

Pore size refers to the diameter of the openings in a material. The size of pores can range from small, such as in clay soil, to much larger, such as in sand. The size of the pores in a material can have a significant effect on its properties.

As the pore diameter decreases, the water is more tightly bound in these pores. The smaller the pore diameter, the higher the suction tension (in cm water column "WS") with which the water is held, corresponding to the conditions in a fine capillary. The pF value (=log cm WS) describes the water retention in the soil in a descriptive form. The higher the pF value, the more "force" it takes to remove the water from the pores. Reference values for this are given in Figure 1: Pore distribution (Blume et al., 2010).. The pF value can be converted into kPA, mbar and other voltage units.

Very fine pores, for example, can only be emptied with the aid of strong negative pressure or heat. This phenomenon is, among other things, responsible for the fact that microorganisms can safely store themselves in the soil even in the case of severe desiccation (sporulation still remains the decisive factor in the survival of microorganisms).

In the table we see the distribution of the pores. We can further divide the coarse pores into wide and narrow coarse pores. Between 10-50mm they are called narrow and >50mm then wide coarse pores. The narrow ones act more like center pores and are a good mix of drainage and water retention. Above 50mm it acts more as pure drainage pores as no water can be held against gravity.

Pore type	Diameter in mm	Waterpressure	pF-value	Function	Aeration	Plant availability
wide	>50	<0,06	< 1,8	Movement of drain and adhesive water	easy	easy
middle	50-0,2	0,06-15	1,8 - 4,2	Water storage (plant available)	hard	medium-heavy
narrow	< 0,2	>15	> 4,2	Dead water (not plant available)	none	none

Figure 3: Pore distribution (Blume et al. 2010)

An important function of pores is their role in water retention. Pores that are large enough to hold water, but not so large that the water drains away quickly, are called "effective pores" or central pores. Materials with a high percentage of effective pores are able to hold more water and retain it for a longer period of time. This can be advantageous in areas with low rainfall or drought.

Pores also play a role in the exchange of gases, such as oxygen and carbon dioxide. In soils, for example, pores are important for air movement and the exchange of gases between the soil and the atmosphere. This is crucial for the growth and health of plants, as they depend on the supply of oxygen for respiration and the uptake of nutrients.

For this reason, as mentioned at the beginning, attention must again be paid to the respective application. This determines whether you need more drainage or more water retention capacity, and depending on this, you have to maintain certain mixing ratios for the soil substances in order to obtain corresponding distributions of pores. The goal is always to maintain approximately the profile of peat, so if I now add very dense materials such as biochar or clay/compost mix, I must add aeration materials to the same degree to maintain the balance.

Our basic material peat has excellent properties for a wide range of applications. The pore distribution shows a left-sided deflection, especially in the central pores. This means that the largest volume proportion of the pores is in the plant-available area. Black peat ( c ) is particularly suitable for this purpose.

 

Figure 4: Pore size distribution of different peat types. (Turunen et al. 2019)

Field capacity and irrigation

Without water, no healthy soil. Without proper watering, the plant's decomposition processes and nutrient supply cannot be maintained. In addition, you can permanently damage your soil if you let it dry for too long.

An important term is the field capacity (FC). It corresponds to the water volume of the soil samples drained to pF 1.8 (=60 cm water column) or taken at corresponding moisture content. This means more or less how much total available water in the soil can be held against gravity in the pores i.e. our maximum available water storage.
According to the classification of pore sizes, the pore range from 0.2 mm to 50 mm is called the usable field capacity (nFK) of a soil, because the contained pore water is available to plants. The water of pores < 0.2 mm is no longer plant-available and is therefore referred to as "dead water" because it would require too much suction from the plant to be used. It is factored out when calculating nFK. The so-called permanent wilting point (PWP) is pF 4.2 or 1000kPA. (Blume et al. 2010). To make the whole thing a bit clearer, you can watch this video

If the PWP is reached during cultivation, the plant dies. However, the point at which wilting begins is different for different plants. Cannabis s., for example, is tolerant and can dry back to high pF values without dying. However, this should only be used to apply targeted stress stimuli, as permanent drought means reduced yield.

Depending on how much % nFK we have filled in the soil, there is a different amount of suction pressure or tension on the root. The drier the soil, the more force the plant has to use to absorb the water.

This suction strength can be measured with a tensiometer and is called matrix potential in soil science. The matrix potential is measured in mbar or kPA. Since little research has been done on this / there are few established values for peat, one must resort to empirical values and anecdotal evidence. These come either from users on the forums or from podcasts such as "Cannabis science and cultivation" by Tad Hussey. One example is an episode from the

The optimum values determined so far also vary from cultivar to cultivar and must finally be determined by the grower himself based on his assessment of the vitality of the plant. The lower the suction tension, the more saturated the soil is with water. In the values we can see that we drive the vegetative phase wetter than, for example, in deliberate stress periods such as the Flower I or Flower III phase in which we want to induce light drought stress.

Figure 5: Water holding capacity under suction stress

However, stress should only be applied when there is full control over the system and the plant, because if the plant is already stressed anyway, we do not want to apply more unnecessarily. (Geelen et al. 2019)

There are ways to quantify this stress, but this requires expensive research equipment and is not suitable for the home user. For commercial cultivators, this is enormously worthwhile for establishing cultivation SOPs for the individual cultivars. However, this requires a research manager or consultant who is knowledgeable in this area.

Here are guideline values so far determined by me and the scene for suction tension in indoor, peat-based living soil systems:

Life phase	Start Veg	Center	End Veg	Flower I: Transition	Flower II: Bulk	Flower III: Maturation
Mbar Range	40-50	40-60	40-80	120-160	60-100	120-160

Table 1: mbar ranges for each phase of growth

To optimally control the degree of saturation, you need a good tensiometer as mentioned above. The best not purely commercially available is the Irrometer SR, which is suitable for light soils, such as our peat mixtures. There are also variants with digital output which can be incorporated into an irrigation controller, but to show this in detail is again beyond the scope.

Cheap alternatives can be found at Blumat or similar manufacturers. These are to be used at about 30cm depending on the depth of the bed. It is best to take the center of the bed, but also measure the entire bed at regular intervals to determine any inhomogeneous water distribution and adjust your watering behavior. Now if you want to use a very fancy/high tech term, you could call this watering behavior organic crop steering, although this also involves controlling the environment actually. It is the supreme discipline and we will discuss in chap. 4. procedure and schedule for the use of Living soil discussed.

Chemical factors

Now that we have considered the physical properties as far as possible, we turn to the chemical properties of soil and plant nutrition. First an overview of the 16 nutrients needed for a productive life cycle in plants, then the pH value in focus, followed by the exception principle of nutrients at the root and finally an explanation of the ion exchange principle at surfaces.

Plant nutrients 101

Like all plants, cannabis requires a balance of macro- and micronutrients to grow and develop properly. Macro and micronutrients are needed for a variety of plant processes, including photosynthesis, protein synthesis, and enzyme function. I recommend (Maathuis and Diatloff 2013; Pandey 2018) as reviews on the topic with in-depth physiological explanations.

For detailed information on plant nutrients specific to cannabis, see the research of Dr. Nitrite Bernstein, (Llewellyn et al. 2023) and (Cockson et al. 2019b) recommended, or for practical videos on the topic, the Greengenesgarden YouTube channel.

Reference values, application instructions and recommended concentrations of the nutrients come in the chapter".3. how to test these components of my soil system", because there are some different adjusting screws and you still have to know them to be able to do something with them.

Macronutrients

There are 6 important macronutrients that cannabis plants need for their growth and development. These include nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg) and sulfur (S), which are required dry at a minimum of 150g/kg of plant material and are therefore also called macronutrients.

Nitrogen

First to the most important ingredient, as it is the basis for all protein production in the plant, which make up the most functional molecules of the plant. It is therefore integral to growth and biomass. It is also a component of nucleic acids (adenine, guanine, cytosine, thymine, and uracil), which form the basis of DNA information and is required for the production of chlorophyll, which is the functional organelle of photosynthesis. (Pandey 2018)

Figure 6: Nitrogen forms and their use in the plant. (Pandey 2018)

The substance is contained in most chlorophyll-containing tissues of plants and in any amino acid-based substances such as peptides/proteins from e.g. blood/horn/soy. There are a variety of organic compounds that carry nitrogen, but most of it is added to the soil in the form of red composts or worm composts.

These organically bound forms such as humic acid (C9H9NO6) are complex and diverse so an exact list would be too much, but there will be an amendment list still in chapter 2.4.5.

When these substances are digested by microbes or mineralized/available through chemical interaction, most of them are present as ammonium (NH4+) or nitrate (NO3-). Annual plants such as Cannabis s. exhibit ammonium intolerance above a certain, cultivar-specific concentration (Esteban et al. 2016). However, up to this concentration, it can be beneficial to supplement some of the nitrogen via NH4+ , because as seen in Figure 4: Nitrogen uptake and utilization in the plant (Li et al., 2017). this again saves a synthesis step and thus energy / ATP, which the plant does not have to expend.

In this figure, one can also see once again the advantage of direct amino acid application, because these can be directly processed into glumatinic acid and used as a basis for the following synthesis steps. (Pandey 2018)

Figure 7: Nitrogen uptake and utilization in the plant. (Li et al. 2017)

Phosphorus

Another important macronutrient for cannabis plants is phosphorus. It is required for the synthesis of DNA and RNA as well as for the production of energy in the form of ATP (adenosine triphosphate). That is, it is both contained in every cell as a cellular template and is the basis for any final energy source of the cell and is considered the currency for every process that takes place in the plant. (Pandey 2018)

Furthermore, it is the most important component of the membranes, because these are literally phosphorus bilayers, an example is the casparic strip, which acts as a separator and filter in the roots. They are also, along with calcium, the most important biomineral in trichomes and are partly responsible for "white ash" due to their white residues during combustion. (Weigend et al. 2018)

Figure 8: Mineral composition of plant trichomes (Weigend et al. 2018)

It is largely taken up as H₂ PO₄^- and is present in the soil either as inorganic, pure phosphorus or organophosphoric form. Amendments used are mainly rock phosphates such as soft-earth rock phosphate or animal products such as manure, guano or bone meal. (Pandey 2018)

A deficiency results in reduced photosynthetic activity and thus is accompanied by reduced efficiency and lower activity of various coenzymes, making the plant vulnerable to biotic and abiotic stress.

Potassium

Due to the high permeability of plant membranes to K+, plants take up potassium quickly. It is characterized by high mobility in the plant and is translocated to younger tissue.

A close relationship between the water channels and the channels for potassium uptake contributes to the maintenance of plant homeostasis, this uptake mechanism is discussed in more detail in chapter 2.2.3.
It is widely distributed in the cytoplasm and serves to maintain ionic balance and osmoregulation in plant cells by neutralizing insoluble macromolecular anions. Its high concentration in cell vacuoles promotes turgor-controlled development of cell elongation; this is also used, for example, in guard cells of stomata. These are pumped full of K+ via ion channels and water then migrates in through osmosis, causing the guard cells to open.

Potassium deficiency reduces turgor and cell size, resulting in reduced leaf area and stem elongation. The deficiency also damages the cell internal pH regulation and impairs the activity of over 50 enzymes, which again ends in susceptibility to biotic and abiotic stress.

Sulfur also protects against dangerous heavy metal deposition via phytochelatins. Excess heavy metals induce the expression of phytochelatin synthase, which catalyzes the production of the low molecular weight polypeptides known as phytochelatins from glutathione.

This substance is supplemented mainly via red composts, worm composts and minerals such as langbeinite. In KNF, water extracts from potassium-enriching plants such as tobacco are used, i.e. direct addition of potassium.

Calcium

Calcium occurs in plant tissue as Ca2+, Ca-carbonate, Ca-phosphate and Ca-oxalate. Which of these forms accumulate in the soil is of immense importance, since, for example, an accumulation of bi carbonates in the soil makes most nutrients insoluble and thus unavailable to the plant. The uptake of Ca2+ is slow, as it is only absorbed by immature root tips. It is essential for root development as can be seen in the figure, here the root orients itself based on the calcium gradient. (Pandey 2018)

Figure 9: Calcium-dependent root growth. (Carol and Dolan 2002)

Uptake is a passive process inhibited by the presence of K+, Mg2+ and NH4+. A small amount of calcium is maintained in the cytoplasm to avoid unfavorable interactions with other nutrient ions (PO4, Mg2+) and deactivation of enzymes. It is also needed for it to serve as a second messenger. The low cytoplasmic calcium concentration (0.2 M) is maintained by controlling Ca2+ flux across cellular and subcellular membranes. (Pandey 2018)

Membrane integrity and thus the resistance of cell walls is directly dependent on the calcium concentration. Callose, which is enriched at the cell junction shafts (plasmodesmata) as a virus-fighting measure, is also based on calcium. This blocks one of the virus/viroid spreading pathways between plant cells.

A recent discovery is role of Ca2+ as a signal transduction molecule. The perception of stress signals causes calcium channels to briefly open and calcium to be pumped into the cytoplasm, increasing its cytosolic concentration. Which then triggers defense mechanisms in the context of PTI (pattern triggered immunity).

It is an immobile nutrient and must therefore be transported in the irrigation water from the plant through the xylem pathway system to the top of the plant. Deficiencies show up first at the tips.

Magnesium

Magnesium is required in significantly lower amounts than other cations such as calcium. This could be due to high cation competition in absorption and a lack of magnesium transporters in the plasmalemma. Low pH and cations such as K+, NH4+, Ca2+ and Mn2+ inhibit Mg2+ absorption. The rate of absorption is slow.

Magnesium has several different functions. The mobility of magnesium in cells is related to its function. The main function of magnesium comes from its position as the core element of the chlorophyll molecule. This organelle is the cornerstone of photosynthesis and when the central atom is missing, logically, photosynthetic performance is severely compromised (Pandey 2018).

Typical amendments for magnesium supply are algal lime or epsom salt (MgSO4-), which still usefully contains sulfur. Depending on the organic association, however, care must be taken with the use of sulfates, as these are sometimes considered "evil" mineral fertilizers.

Sulfur

Sulfur is present as SO₄^- i.e. sulphate, the most typical forms are gypsum (calcium sulfate), which is additionally an excellent pH-neutral calcium supplier, and organic compounds such as in composts.

Sulfide reduction in plant metabolism results in the incorporation of sulfide into a variety of different molecules, including the amino acids cysteine and methionine, coenzymes, and secondary metabolites. Cysteine and methionine are both essential components of plant proteins. These amino acids are particularly important because animals and humans are unable to break down sulfur and depend on plants to meet their nutritional needs. This is why an adequate supply of sulfur is important in any plant. Glutathione, the most important detoxification enzyme, is also based on sulfur compounds (cysteine) (Pandey 2018)

Sulfur protects against dangerous heavy metal deposition via phytochelatins. Excess heavy metals induce the expression of phytochelatin synthase, which catalyzes the production of low molecular weight polypeptides known as phytochelatins from glutathione. (Pandey 2018)

In the case of cannabis, the point of increased secondary metabolite synthesis is of particular importance, as these particularly favor VSC (volatile sulfur compounds) accumulation. These substances are responsible for the garlic odor in GMO, for example. However, there are many different ones and they can be most attributed to the gas/garlic notes if you don't differentiate between them. One of the best papers on this topic is. (Oswald et al. 2021).

Figure 10: VSC analysis of Bacio Gelato (Oswald et al. 2021)

Carbon

Not one of the classically valued plant nutrients, but essential is carbon. This is absorbed through the air as CO2 and acts as a source of carbon for glucose production (sugar) in photosynthesis. However, it also has other important roles in the soil. First, every organic matter and many important chelating agents (availability increasing) such as humic acid are carbon based. It serves as a carrier for most nutrients and is the cornerstone of all life.

Storing atmospheric CO2 from the air in the plant and soil after cutting, is the key to reducing greenhouse gases in the atmosphere.
This process is called CO2 sequestration and through careful calculation and compensation areas can thus compensate for the emissions produced (fertilizer/tractor/processing). This is the foundation of sustainable agriculture and the only way to date to truly efficiently remove climate-changing gases from the air.

In addition, this will become a valuable market basis for emissions trading in the future. It is all calculated with a carbon balance and additional points/deductions are given for certain methods. A good review on the methodology was co-published by Prof. Dr. Hülsbergen, from whom I was also briefed on this topic (Küstermann et al., 2008)

Figure 11: Formula humic acid (Stevenson 1994)

Micronutrients

Anything less than 100 g/kg plant mass dry is called a micronutrient, these are iron, boron, molybdenum, copper, zinc and chlorine. Especially molybdenum, boron and iron are common deficiencies in cannabis so special attention should be paid here (Cockson et al. 2019a). Most micronutrient effects relate to use as a co-factor in enzymes or are involved in the mitochondrial electron transport chain.
Boron takes

Figure 12: Copper-dependent enzymes (Pandey 2018)

Figure 13: Manganese-dependent enzymes (Pandey 2018)

Figure 14: Molybdenum-dependent enzymes. (Pandey 2018)

Figure 15: Various iron-dependent enzymes. (Pandey 2018)

Additives / Strengtheners

Silicone

For plants to maintain their structural integrity, they need the micronutrient silicon. It contributes to the formation of cell walls and the control of plant growth. Together with calcium, this nutrient gives plants their real strength. It is crucial because bud rot can easily penetrate and develop in the large buds, especially under high intensity conditions (1000umol/m2*s, 1200ppm CO2, etc.). In addition, it reduces the need for binding and helps strengthen very heavy branches that might otherwise break. This is especially important for the original GG #4 cut, which naturally forms large inflorescences and flies all over the place. In terms of cannabis, some studies have shown that certain micronutrients may have specific functions in cannabis plants. For example, a study published in the Journal of Plant Nutrition and Soil Science found that zinc deficiency in cannabis plants can lead to reduced growth and cannabinoid production (Landi 1997).

There are other substances that influence plant nutrition but are not directly absorbed as nutrients. They have partly positive, but also negative effects.

Sodium

This element is contained in most nutrient carriers such as kelp or mussel shells, which come from the sea. It is not directly needed by the plant and an accumulation is even harmful for the plant, because the substance displaces the other nutrient cations with its positive charge.

Chlorine

Maintenance of turgor and osmoregulation is an essential function of chlorine in plants. The osmoregulatory functions of chlorine include its involvement in turgor-regulated cell development and stomatal function. Chlorine accumulates in large amounts in root and shoot tips, where it is involved in turgor-driven development of cell processes. Nevertheless, too high levels should be avoided to limit the oxidizing effect on bacteria and micro life.

In most cases, enough chlorine is introduced into the system via composts or the water so that it does not have to be supplemented extra.

Conversion formulas and chemical nutrient forms

It should be noted that in the Amendments, the contents are mostly in their bound form such as P O₂₅ .

The conversion factor between P2O5 and P depends on the molecular weight of the respective compound.
The molecular weight of P2O5 is 141.94 g/mol, and it contains two phosphorus atoms.
The molecular weight of P is 30.97 g/mol.
To convert P2O5 to P, multiply the P2O5 value by 0.4364 (or divide by 2.29).
This means that 1 gram of P2O5 contains 0.4364 grams of P.
Therefore, to convert from P2O5 to P, you can use the following formula:

P = (P2O5 * 0.4364)

Or

P2O5 = (P / 0.4364)

Here is a list of all plant available forms of nutrients, you can calculate the pure amount as in the example above.

1. Nitrogen (N)

• Nitrate (NO3-)
• Ammonium (NH4+)

1. Phosphorus (P)

• Orthophosphate (H2PO4- and HPO42-)
• Mono- and diesters of phosphoric acid (organic P)

1. Potassium (K)

• Potassium ions (K+)

1. Calcium (Ca)

• Calcium ions (Ca2+)

1. Magnesium (Mg)

• Magnesium ions (Mg2+)

1. Sulfur (S)

• Sulfate (SO42-)
• Elemental sulfur (S) in oxidized soils.

1. Iron (Fe)

• Iron (Fe2+)

1. Manganese (Mn)

• Manganese ions (Mn2+)

1. Zinc (Zn)

• Zinc ions (Zn2+)

1. Copper (Cu)

• Copper ions (Cu2+)

1. Boron (B)

• Borax (Na2[B4O5(OH)4])

1. Chlorine (Cl)

• Chloride (Cl-)

1. Molybdenum (Mo)

• Molybdate (MoO42-)

Deficiencies in these elements result in losses in general biomass and decreases in harvest index. To quantify the impact have (Llewellyn et al. 2023) laid out a very extensive study that measured the impact.

Figure 16: Fresh weight (FW) of detached aboveground tissue, harvest index (inflorescence FW/stalks and leaves FW), and dry weight (DW) of roots from each nutrient treatment. (Llewellyn et al. 2023)

Figure 17: Change in cannabinoid composition under specific nutrient deficiencies. (Llewellyn et al. 2023)

However, these losses are specific to the location of the organ, as plant nutrients are either mobile or immobile within the plant itself.

The concept of mobile and immobile nutrients refers to how easily these nutrients can move within a plant after being taken up by the roots. Mobile nutrients can easily move from older to younger plant tissues, while immobile nutrients remain in the tissues where they were originally taken up.

Mobile nutrients include nitrogen (N), phosphorus (P) and potassium (K), which are important macronutrients for plant growth. These nutrients can be easily transported within the plant via the phloem, a specialized vascular tissue that transports nutrients and sugars through the plant.

Immobile nutrients, on the other hand, include calcium (Ca), sulfur (S) and many micronutrients such as iron (Fe) and zinc (Zn). These nutrients are not easily transported within the plant and remain in the tissues where they were originally taken up.

Nutrient mobility can have important effects on plant growth and development. For example, if a plant is deficient in a mobile nutrient such as nitrogen, it will transfer nitrogen from older leaves to younger leaves to maintain growth. This can cause older leaves to turn yellow or brown because they no longer contain nitrogen.

In contrast, a deficiency of an immobile nutrient such as calcium can result in stunted growth or malformations in affected tissues because the nutrient cannot be readily transported to other parts of the plant to support growth.

This is immensely important in order to be able to isolate deficiencies at an early stage and to initiate appropriate re-fertilization. One of the best papers in the field, and one of the first in medicinal/current cultivars, is that of (Cockson et al. 2019b).

The PH value and the influence on the ionic form of the nutrients

To ensure maximum growth and development, it is important to ensure that cannabis plants receive the right mix of each of these 16 nutrients. Cannabis plants have different nutrient needs depending on their stage of development, the type and pH of their soil, and the presence of other nutrients. To determine the nutrient content of their soil and adjust their fertilization practices accordingly, growers can use soil tests and plant tissue analysis because the charge and shape of nutrients is affected by the milleu.

In most cases, the nutrients mentioned above are not present in neutral form or are already ions themselves. The charges play an important role in the uptake of nutrients by the roots. Up to now, the uptake capacity of nutrients has been determined on the basis of the table of (Peterson 1982) was used.

Figure 18: Availability nutrients in a peat-based and enriched system. (Peterson 1982)

Recent studies by (Hartemink and Barrow 2023) however, show that this is not so easily applicable. There are still so many interactions at play, such as the various effects of microbial community changes due to exudates, that one can only estimate these PH ranges for inert systems. Even this is still imprecise; influences of root exudates on PH and mineralization rates would also have to be determined to make accurate statements. But this is already beyond the scope of what is possible in home cultivation. That is, especially in living soil systems, neither the PH of the water nor that of the soil can provide accurate information about nutrients. (Hartemink and Barrow 2023).

Roughly, this is nevertheless to be considered in cultivation. Because a soil PH of e.g. > 9, suffers quite certainly from a too high accumulation of calcium carbonate or similar substances. Here, the uptake of nutrients is severely restricted, especially that of micronutrients, which are essential for the regulation of almost all processes and for enzymes. For the pure mineral availability of nutrients, 5.5-5.8 is optimal as it is also used in mineral, commercial cultivation.

However, a pH below 6.0 then becomes problematic again for the microbiome, as below 6.0 it partially switches to an anaerobic, heterofermentative milieu, which we want in certain phases (Stretch/Flower I), but not in most phases. Here we want to maintain a pH of 6.0-6.8, as this is where most of the beneficial microorganisms live, cf. 2.3.1 Micro Flora.

If you now want to create an optimal, cultivar-specific recipe, you can set the p.H. higher or lower depending on the nutrient requirements of the cultivar. My personal sweet spot is 6.5 in the vegetative phase, which then decreases to 6.3 in the course of flowering. However, as mentioned above, this is only an estimate, since microbial life has to be taken into account. In the next chapter, we will also see that the plant/microbe interaction has a considerable influence via the release of sugars and the subsequential propagation of special microbes by the plant. Thus, the plant controls most of the processes and we give it more or less only the tools to be able to develop independently, optimally.

The uptake of nutrients at the roots

Plants absorb nutrients through their roots in the form of ions. These ions arrive at the root via 3 mechanisms. These are interception, mass flow and microbial interaction, the first of which refers to root penetration and subsequential uptake. The second mechanism (mass flow/mass flux) refers to the transport by means of water, which directly flushes ions into the root, this can also be disadvantageous if a too high dissolved ion concentration (too high EC value) is reached in the nutrient solution of the soil. Then the plant can no longer defend itself against the nutrient, so to speak, and is overfertilized / force-fed. Unfortunately, this does not result in a better tasting end product as in the case of foie gras, but rather the opposite and it "burns" the plant. That's why we have to test in tests (chap. 3. how to test these components of my soil system) we always have to pay attention to the mineralization rate, this is determined by comparing stock tests (Merlich III) with solution tests (saturated paste) and measuring the % solution/unit of time. This is of course only an estimate, as we do not have real time data on the mineralization rate. If this is too high, the EC value of our soil solution will also be too high and the plants will die.
The third way nutrients get into the plants is the interaction of microbes (mostly arbuscular endomychoraceae) with the roots. Exact mechanisms and preferred nutrients for this uptake mechanism are discussed in chap. 2.3.2.2 Fungi (PGPFs) will be discussed.

The movement of ions in and out of plant cells during interception is regulated by the concentration gradient, which is the difference in ion concentration between the inside and the outside of the cell. When the ion concentration on the outside of the cell is higher than on the inside, ions flow into the cell to equalize the concentration. This movement of ions into and out of the cell is facilitated by ion transporters, which are proteins that help move ions across the cell membrane. (Wang et al. 2006)

In addition, more highly charged substances such as Mg2+ are absorbed more strongly than K+ with one charge. This can lead to a displacement effect at the root if the concentration of individual substances is too high, since only the strongly charged Mg2+ are taken up, but the weak K+ firstly cannot get through and secondly are repelled by the Mg2+ since they have the same charge. This shows the immense importance of balance in the system, MGMs are like ballet and not like a strongman competition, harmony counts and not much helps much. Especially the cations need to be kept in mind, because while the microbes can balance a lot, they also can't balance everything. We create, as mentioned before, the perfect toolbox for the plant and if there are too many nails, the plant will not find any more screws, to put it a little more vividly. More about this in chapter 2.2.4 CEC and AEC - Cation and Anion Exchange Capacity and Base Saturation

And here again a scheme for nutrient uptake based on a root cross section

Figure 19: Schematic of nutrient uptake based on a root cross-section. (Wang et al. 2006)

We see here on the left side the passive transport. This is mainly for nitrogen derivatives and other charged nutrients. The plant does not have to do any "work" here, as it flows in along the natural H+ gradient. I.e. the charge within the root fits so that the nutrients can be absorbed and the anion/cation balance still fits.

Active transport is used when the work-free uptake path is blocked. Either H+ is expelled from the root during cation uptake, because otherwise there is too much + charge in the root. These channels are called antiporters.

For anion uptake against the natural gradient, a symporter channel is used. This channel packs the - charged particle together with an H+ atom in order to keep the balance and not to disturb the charge equilibrium. In summary, the plant must always maintain a charge balance and exchanges either equal or different charges with the soil. The plant can also control the internal charge composition, but of course always prefers to do as little work as possible itself.

For this reason, there are also higher-molecular, organic compounds such as amino acids, which can be absorbed and thus cost the plant less build-up energy (ATP) than low-molecular nitrate. However, these must be applied directly through the leaf, because in the soil they are pre-digested by the microbes and are then pure nitrate again, which, then must first be built up again. I.e. amino acid fertilization via the soil is relatively useless for the plant itself, but you can use it as food for microbes, but I would not take expensive pure aminos, but compost or fresh green cuttings. Here again an overview of the nitrogen cycle of the plant, which starts with glutamine from nitrate and thus forms the basis for all amino acids on this planet. There are almost no other organisms that can do this step. Without them, there would also be no humans or animals from meat (Tegeder and Rentsch 2010). Which transporters are responsible for which function, we see in Figure 10&11.

Figure 20: transporter modus operandi pt1 (Sasse et al. 2018)

Figure 21: Transporter modus operandi pt 2 (Sasse et al. 2018)

We have already looked at the uptake of plant nutrients at the chemical level, but now we are still missing the interaction of plant and microbes mentioned above. Plants can excrete certain polysaccharides / monosaccharides (multiple / simple sugars) from their roots, which then serve as specific food for specified microbes. I.e. the plant can independently, if it has all the necessary substances / is healthy, control the composition of the microbiome.

If the plant now requires more phosphorus, for example, sugars are exported to the rhizosphere by means of the SWEETS or MFS transporters, as can be seen in Figure 10. In addition, Arabidopsis plants (model organism) deficient in phosphate produced more coumarin and oligolignol, poplar (Populus tremula) treated with heavy metals produced more organic acid, and wheat deficient in zinc produced more phytosiderophores. Differential exudation is one possible pathway by which plants can alter their interactions with microorganisms, as shown by the relationship between exudation patterns and rhizobiome variation in eight Arabidopsis lines. With this and Figures 10&11, we see that plants not only exude these sugars for microbial interaction but can also exude organic acids (elimination nutrients & pH regulation), amino acids and other chemicals. This has hundreds of regulatory effects. The best systematic review on the subject can be found at. (Sasse et al. 2018)

More detailed information on the organisms is presented in chap. 2.3.1 Micro Flora is dealt with.

Up to 20% of the plant's total sugars produced (photosynthesis/Calvin cycle) are spent on these interactions when needed. (Sasse et al. 2018) This number must be reduced so that we cover the basic needs with mineral nutrients, which do not require microbial breakdown, and let the microbes cover the specific characteristics of the cultivars. I.e., balance is again of paramount importance. We do not want to have everything produced by the microbes, but only the fine tuning of the nutrients. Basic needs are provided by minerals/mineralized nutrients. This is also the reason for the optimal expression of the

CEC and AEC - Cation and Anion Exchange Capacity and Base Saturation

Now we have looked at both the soil water supply and what nutrients can dissolve in that water. Now we only need information about the nutrient storage capacity of our soil.

The cation exchange capacity (CEC) describes the ability of a material to exchange positive charge carriers (cations). The counterpart to this is the anion exchange capacity (AEC), which describes the ability of a material to exchange negative charge carriers (anions).

The CEC and AEC of peat are usually measured in milliequivalents per gram (meq/g). The CEC and AEC of peat depend on several factors, such as the chemical composition of the peat, the size and shape of the peat particles, and the conditions under which the exchange takes place.
Unlike in the grown soil of sand, silt and clay, our peat-based system without amendments is still quite meager in terms of plant nutrient storage capacity. In normal soil, there are clay and other mineral compounds with positive/negative charged surface for this purpose.

Figure 22: Cation exchange capacity of different materials (Andrews 2013)

Peat contains many quartz sand grains and organic components, both of which are electrically charged. The organic components have a negative charge, while the quartz sand grains have a positive charge. When peat comes into contact with water, the cations and anions from the solution can bind to the electrically charged particles in the peat. This exchange process is called adsorption.

Soil additives such as minerals (zeolite, langbeinite, etc.) as well as organic substances such as humus/compost improve the storage capacity of the soil and are therefore added. They therefore contribute not only to the filling per se i.e. the pure nutrients, but also to the overall storage capacity of the soil.

The percentage of filling of CEC/AEC is called base saturation and depends on the respective cations/anions already mentioned in 2.2.1 and on the PH milieu. (Blume et al. 2010)

There is no "optimal" value for the cation exchange capacity (CEC) of peat in cannabis growing systems, as the ideal CEC depends on many factors, including the type of cannabis plant, the nutrient requirements of the plant, and the conditions of cultivation. In general, a higher CEC is considered beneficial because it can help ensure that more nutrients are absorbed by the plants. However, too high a CEC can also result in too many nutrients being locked up in the plant, which can lead to a nutrient deficiency

It is measured in meq/100g (milliequivalents/100g). The individual substances and their CEC can be found in chapter 2.4. These milliequivalents can be converted into mg/kg with the respective conversion factor of the specific elements:

Table 2: CEC conversion factors (Local Land Services (NSW Government) 2020)

Cation	mg/kg or ppm(A)	Conversion factor (B)	meq/100g (A/B)
Ca2+	582	200	2,91
Mg2+	168	122	1,38
K+	59	390	0,15
Na+	27	230	0,12

To calculate the full CEC, the meq/100g of aluminum and the free hydrogen must still be added. However, mostly attention is paid to the four main components, since free hydrogen occurs in small amounts (at appropriate pH of 6.2-6.8) and in a healthy soil the aluminum should not be present in high amounts either way. (Local Land Services (NSW Government) 2020)

The CEC can be filled with the main cations in Table 1 in various ratios. The optimal values used today are mainly based on the works of (ALBRECHT 1959]. Different crops and different setups must of course always be slightly adjusted according to conditions. For example, heavy soils need more calcium than magnesium in relation to lighter soils. This is due to the coarser structure of calcium and the resulting additional pores.

Three reference values are used, once from (Logan Labs 2023)once from (ALBRECHT 1959) and once from AgPHD on Youtube, which I can only recommend to everyone.

Table 3: Comparison of different building saturation reference values

Cation/Method	Albrecht	Logan Labs	AgPHD
Ca2+	68-70%	60-70%	65-80%
Mg2+	10-12%	10-20%	12-20%
K+	4%	2-5%	4-8%
Na+	As low as possible	As low as possible	As low as possible

Now, to convert the optimal values into PPM nutrient, use the formula

CEC x nutrient specific value x selected % base saturation

25 (CEC for example soil) x 400 (value for calcium) x 70% (Albrecht %rate for calcium)
= 7000kg/ha = 0.7 kg/m² = 700g/m2 calcium per m2 bed
7000kg/ha / 2.24 = 3125mg/kg soil (Local Land Services (NSW Government) 2020) l
=> optimum value

An example of how to arrive at the required fertilization quantity with a real soil test can be found in chapter 3.2.5 Cations and the base saturation

The calculated value is unfortunately in kg/ha so difficult to adapt to indoor beds. However, you can roughly calculate and estimate the A horizon with 0.5m and then calculate the ha down to m2 bed.
The nutrient specific values of the cations are:

Calcium: 400

Magnesium: 240

Potassium: 780 (Logan Labs 2023)

Biological factors / The Soil food Web

As "Soil Food Web" is usually described in the popular forums the complex ecosystem in the soil, which consists of different species of microorganisms, fungi, worms, insects and other animals. This term was popularized and spread by Dr. Elaine Ingham, and current science is also turning and giving more and more importance to the Soil food web.

Living organisms interact with each other to form a dynamic network of relationships in which nutrients and energy are diverted in a variety of ways. The Soil Food Web is considered the vitality of the soil and is important for soil health, and through the soil affects nutrient supply, resistance to pests, water uptake and the possibility of plant growth.

The cycling aspect becomes even more useful to us as genera such as bacteria are eaten and digested by larger creatures such as the protozoa or worms, which then provides the nutrients mineralized for the plant when excreted. When digested by worms, additional nutrients are even chelated by humic acids.

This cycle goes through all stages until it finally includes macro creatures like birds, which we unfortunately do not have in indoor cultivation, but these are simulated by digging up / mechanically killing the soil / its creatures, so it is almost a complete cycle.

Figure 23: The Soil Food Web - Scheme. (Natural Resources Conservation Service 2023)

We now take a close look at individual parts and clarify what use they have in the horticultural sector.

We can further divide these organisms into micro fauna and micro flora, where fauna is the current animal diversity and flora is the current plant diversity.

Micro Flora

Bacteria

This smallest participant of the microfauna is the bacterium. There are an enormous number of strains of them (this is the correct use of strain, cannabis has varieties) and many have beneficial properties for plant growth. This group is called PGPR (plant growth promoting) bacteria.

These have direct and indirect effects on the plant, here is a list of the processes they favor. Those interested in more detail can get a rough overview in the book series Teaming with microbes (Lowenfels and Lewis 2016) or get a very deep, accurate one in the review (Goswami et al. 2016). The review by Goswami et al. contains all the references needed for the individual proofs, actually these should be listed individually for a perfect citation style, but I don't have the time, please just look in the respective chapters.

Direct mechanisms:

• Biological nitrogen fixation

Biological nitrogen fixation is the process by which atmospheric nitrogen gas (N2) is converted to an ammonium form (NH4+) that can be used by plants. Since nitrogen is an essential component of plant growth but is not readily available in most soils, this process is critical.

Nitrogen fixation is the method used by PGPR bacteria to fix nitrogen, including Rhizobium spp. and Bradyrhizobium spp. This process takes place in specialized organs known as nodules, which develop on the roots of some plants, such as legumes. With the help of an enzyme, nitrogenase, the PGPR bacteria in these nodules convert nitrogen gas into a usable form.

• Solution of the phosphorus that is not available to plants.

The process of phosphate solubilization makes phosphate (PO4 3-) more soluble and thus more available to plants. Although phosphate is an important plant nutrient, it is often present in soils in an inaccessible form, e.g. as inorganic phosphate.

Inorganic phosphates can be dissolved by PGPR bacteria such as Bacillus and Pseudomonas species by producing organic acids such as citric and malic acids. The bacteria release these organic acids, which can chelate with the inorganic phosphates and increase their solubility in water. This allows the plants to absorb the phosphates more easily and use them for their growth and development.

Pseudomonas and Bacillus species that dissolve organic phosphorus are able to remove phosphorus from phytate (Found in manure and compost) and other phospholipids. The inorganic phosphates are then available to the plants after the bacteria have degraded the organic forms of phosphorus.

Again, this is solved by the excretion of citric and malic acid compounds. Other mechanisms such as proton excretion through ammonium (NH4+) accumulation is also possible, but there is only a limited difference in the way the methods work, as the organic acids also only act as proton donors. (Park et al. 2009)

• Phytohormones production

Phytohormones are also synthesized by PGPRs. These serve as plant growth regulators, or PGRs, which help control plant growth. Depending on the desired effect, a different type of PGPR can either be grown naturally and supplemented or isolated in the laboratory and added. This can be used, for example, to induce a compressive effect by cytokinins.

The following is a list of phytohormones produced by various PGPRs:

• Auxins: PGPRs such as Pseudomonas fluorescens, Pseudomonas putida, and Rhizobium leguminosarum have been found to produce auxins responsible for cell elongation and differentiation. They also promote lateral root development and fruit ripening.
• Cytokinins: PGPR such as Bacillus amyloliquefaciens, Bacillus subtilis and Azospirillum brasilense have been found to produce cytokinins, which are responsible for cell division and the development of roots, shoots and leaves. They also promote the development of lateral roots, the ripening of fruits and the development of chloroplasts.
• Gibberellins: PGPR such as Pseudomonas fluorescens, Pseudomonas putida, and Rhizobium leguminosarum have been found to produce gibberellins, which are responsible for cell elongation and differentiation. They also promote seed germination and shoot growth.
• Abscisic acid: Some PGPR such as Azospirillum brasilense produce abscisic acid, which is responsible for triggering dormancy and seed maturation and promotes plant tolerance to environmental stress.
• Jasmonates: Some PGPR such as Pseudomonas syringae have been reported to produce jasmonates, which are phytohormones involved in plant defense response.

Indirect mechanisms:

• Siderophore production

Siderophores are tiny, iron-binding substances formed by microorganisms such as specific bacteria that help them absorb iron from their environment. To help absorb iron from the soil, several plant growth promoting rhizobacteria (PGPR) are capable of forming siderophores.

• Pyoverdins, a substance produced by Pseudomonas spp. that can dissolve iron from iron-containing minerals such as iron oxides and hydroxides,
• The production of bacillibactin by Bacillus spp. allows bacteria to chelate and utilize iron.

By forming siderophores that can also dissolve other micronutrients from the soil, some PGPR have also been shown to affect the availability of other micronutrients such as zinc and manganese, which are comparable to iron in terms of solubility and uptake by plants. (Płociniczak et al. 2013)

• Chitinase and glucanase production by PGPR.

Chitinase and glucanase are both types of cell wall degrading enzymes produced by plant growth promoting rhizobacteria (PGPR). Chitinase is an enzyme that degrades the chitin component of fungal cell walls, while glucanase degrades the beta-glucans found in the cell walls of various organisms, including fungi and bacteria.

An example of a bacterium that produces chitinase is the soil-dwelling Pseudomonas aeruginosa, which has been shown to produce chitinase when growing in the presence of chitin. Another example is Bacillus subtilis, which is known to produce both chitinase and glucanase.

When PGPR produce chitinase and glucanase, they can degrade the cell walls of pathogens such as the fungus Fusarium and other potential plant pathogens that have chitin or beta-glucans in their cell walls. This can lead to suppression of pathogenic infections in the roots and rhizosphere of plants.

In addition, these enzymes can also degrade the cell walls of non-pathogenic microorganisms and organic matter in the soil. This allows plant-available nutrients such as phosphorus, nitrogen and other micronutrients to be released from the degraded cell walls, which can be utilized by the plants.

Furthermore chitin fragments produced by chitinase can induce systemic acquired resistance (SAR) in plants, which is an important mechanism of plant immunity to pathogenic infections and receives its own chapter. (Chet et al. 1990)

Overall, the production of chitinase and glucanase by PGPR is a key mechanism by which these bacteria can promote plant growth and health by increasing the availability of nutrients and suppressing pathogenic infections.

• Antibiotic production through PGPR

In a process known as "antibiotic production by plant growth-promoting rhizobacteria" (PGPR), certain bacteria produce substances with antibacterial properties. These substances can promote plant development and nutrient uptake while helping to protect plants from disease.

Biosynthesis and secretion are the two main processes by which PGPR antibiotics can be generated. In secretion, bacteria release the antibiotic into the environment, whereas in biosynthesis, bacteria actively synthesize the antibiotic in their own cells.

The bacterium Streptomyces griseus is an example of a PGPR that synthesizes antibiotics. Numerous antibiotics, such as griseofulvin and streptomycin, are produced by this bacterium. Another example is the bacterium Pseudomonas fluorescens, which is known to produce the antibiotic chemicals phenazine-1-carboxamide (PCN) and pyoluteorin (Plt). (Hammer et al. 1997)

• Induced systemic resistance

Induced systemic resistance (ISR) is a phenomenon in which the application of certain microbes, such as plant growth promoting rhizobacteria (PGPR), can activate a plant's natural defense mechanisms, resulting in increased resistance to pathogens. This is an important strategy for disease control in crops, as it allows plants to be protected without the use of chemical pesticides.

There are several mechanisms by which PGPR can induce systemic resistance in plants. One of the most important mechanisms is the production of signaling molecules known as elicitors of systemic acquired resistance (SAR), such as salicylic acid (SA) and jasmonic acid (JA). These molecules are produced by PGPR and can activate the plant's SAR response, resulting in increased resistance to pathogens.

Another mechanism is the production of enzymes such as chitinases, beta-1,3-glucanases and peroxidases. These enzymes are known to break down the cell walls of pathogens, making it difficult for them to infect the plant. In addition, the chitin fragments produced during the disruption are recognized as PAMP (pathogen associated molecular pattern). This is a form of marker by which the plant initiates its active defense mechanisms (PTI = pattern triggered immunity). (Couto and Zipfel 2016)

An example of a PGPR strain known to induce systemic resistance by triggering SAR is the bacterium Pseudomonas aeruginosa. This bacterium produces a number of molecules that can activate SAR, including SA and JA. Another example of a PGPR strain known to induce systemic resistance by producing enzymes is Bacillus subtilis, which produces chitinases, beta-1,3-glucanases, and peroxidases. (Goswami et al. 2016)

• PGPR modulates plant stress markers under abiotic stress.

Abiotic stress is any stress to which a plant is exposed that is not biological in nature, such as drought, high salinity, or harsh temperatures. These stressors can alter the expression of certain genes, resulting in stress indicators that can be used to measure the extent of stress.

Under abiotic stress conditions, PGPR can alter plant stress indicators through a number of different methods. The formation of molecules that promote plant growth, such as indoleacetic acid (IAA) and cytokinins, is one of the key processes. These substances can help mitigate the effects of stress by promoting plant growth and protecting the plant from stress-related damage.

Another mechanism is the production of enzymes such as dehydrin, proline, and the antioxidant enzyme superoxide dismutase (SOD), which can help protect the plant from stress-related damage by stabilizing the cell membrane, preventing protein denaturation, and reducing the production of reactive oxygen species (ROS) in the plant.

An example of a PGPR strain known to modulate plant stress markers through the production of plant growth-promoting compounds is the bacterium Bacillus subtilis. This bacterium is known to produce IAA and cytokinins that can promote plant growth and protect the plant from stress-related damage. Another example is the bacterium Pseudomonas putida, which is known to produce the enzymes dehydrin and proline, which can help protect the plant from stress-related damage by stabilizing the cell membrane, preventing protein denaturation, and reducing the production of ROS in the plant (Niu et al. 2012; Goswami et al. 2016; Rasool et al. 2013).

• Preparation of 1-aminocyclopropane-1-carboxylic acid deaminase.

1-Aminocyclopropane-1-carboxylic acid (ACC) deaminase is an enzyme that converts ACC, a precursor of the plant hormone ethylene, into ammonia and alpha-ketobutyrate.

The production of ACC deaminase by PGPRs is a well-documented mechanism in the regulation of plant growth and stress responses. ACC deaminase breaks down ACC, a substrate of the plant hormone ethylene, into ammonia and alpha-ketobutyrate. This effectively lowers ethylene levels. Ethylene is a hormone known to have an inhibitory effect on plant growth and is also associated with stress responses. (Etesami et al. 2015)

An example of PGPR that produce ACC deaminase is Pseudomonas spp. This group of bacteria produces ACC deaminase and has been shown to have growth-promoting effects on a variety of plant species.

Another example is the bacterium Burkholderia spp. which reportedly produces ACC deaminase and has been shown to promote growth and improve plant tolerance to various abiotic stresses such as drought and high salt concentrations.

Archae

Microorganisms with a single cell belong to the archaea domain. They are a diverse group of living organisms that differ both chemically and genetically from bacteria and eukaryotes (the other two domains of life).

Archaea are found in a variety of environments, including harsh environments such as salt pans, hot springs, and deep-sea hydrothermal vents. They are also found in more temperate environments such as the soil and the sea.

Both bacteria and archaea are important components of the soil microflora and have a significant impact on soil processes that influence plant growth and health. Both types of microbes can influence plant growth by decomposing organic matter and releasing nutrients that can be taken up by plants, promoting the development of favorable soil structure, and acting as a barrier against pathogens.

One of the main differences between archaea and bacteria is the process of energy production. While many bacteria use oxygen in respiration to produce energy, archaea are often anaerobic and produce energy through a process known as methanogenesis. Methane is the result of the metabolism of methanogens, which are archaea. This can significantly affect the carbon cycle in soils, and methanogenesis may even contribute to increased soil fertility, according to some research.

The ways in which bacteria and archaea interact with plants also differ. While some bacteria have been shown to have direct reciprocal relationships with plants, for example by fixing nitrogen in nodules on the roots of legumes or by supporting plant growth through the production of appropriate chemicals, this is generally not the case for archaea. Archaea may also be involved in the development of biofilms on plant roots that can help protect plants from disease. Some archaea have been shown to produce volatile organic chemicals such as methanol or formaldehyde, which can stimulate plant growth by improving nutrient availability.

They are also involved in the sulfur cycle. They oxidize sulfur and produce sulfuric acid with it, which can dissolve minerals from stones. Calcium, for example, can be converted to calcium sulfate if calcium-containing compounds are available. This substance is commonly referred to as gypsum and provides both good aeration due to its pore structure and a source of nutrients when it decomposes. (Lin et al. 2010; Naitam and Kaushik 2021)

Micro Fauna

Before we go into the exact tasks of soil organisms, we need to establish a classification according to their tasks.

The so-called necrotrophs, feed on dead or decaying substances. They are critical to the decomposition process that helps return nutrients to the ecosystem. They actively kill the plant to get at the dead tissue.

Microbes that feed on living things are called biotrophs. They often form symbiotic partnerships with plants, animals and other species, each receiving a benefit in exchange for the other.

Depending on the resource supply, facultative microorganisms can exist in either living or dead substances. Depending on the environment, they can occur as necrotrophs or biotrophs.

Microorganisms that feed only on substances that are already dead or decaying are called saprophytic microorganisms. They are critical to the decomposition process that helps return nutrients to the ecosystem. Bacteria, fungi, and various species of algae are some examples of saprophytic microorganisms. (Agrios 2005)

Algae

The influence of algae and their produced substances in the soil are already known since the 1960s and conditional researches have already been established. The review by (Dmytryk and Chojnacka 2018) gives a very good overview of their suitability as producers of biostimulants and use as plant fortifiers.

Aquatic organisms known as algae are a diverse category that includes both simple unicellular organisms and complex multicellular forms. They are distinct from plants, animals, and fungi and are classified in the Protista kingdom. There are many different types of habitats in which algae occur, including freshwater, marine, and terrestrial ecosystems. They can produce their own food because they are photosynthetic, meaning that light energy is converted into chemical energy during the process of photosynthesis.

Algae play an important role in the decomposition of organic waste and nutrient cycling in the soil-compost cycle. By secreting enzymes that break down complicated compounds, algae in the compost pile can accelerate the decomposition of organic material.

They can also absorb and store nutrients that are important for plant growth, such as nitrogen, phosphate and potassium.

Attached is a list from the paper (Dmytryk and Chojnacka 2018) for overview, I will not go into detail about each substance here.

Figure 24: The effect of poly- and oligosaccharides produced by algae when applied to plants. (Dmytryk and Chojnacka 2018)

Figure 25: The effect of phytohormones and hormone-like compounds when applied to plants. (Dmytryk and Chojnacka 2018)

Fungi (PGPFs)

The next huge component of soil microorganisms are fungi. Here, there is also a large number of different mechanisms of action. To cover the whole topic completely will again be very difficult in the short time available. Again, I recommend a review article:

A diverse group of fungi known as plant growth-promoting fungi (PGPFs) interact symbiotically with plants. They colonize roots and are critical for plant development, nutrient uptake, and tolerance to biotic and abiotic stresses. Many strains, such as the Glomeromycota, Ascomycota, and Basidiomycota, contain PGPFs.

Glomeromycota

The phylum Glomeromycota is known to play an important role in the development of arbuscular mycorrhizal (AM) associations with plants.
There are two types of mycorrhizal fungi, once endo and ectomycorrhiza. We need the endo variety as it forms symbiotic bonds with grasses and crops. The ecto strains are found on trees such as the common boletus in spruce. They settle on the surface of the roots and can form fruiting bodies.

In contrast, the arbuscular endo-mycorrhizal fungi we need are found in an intermediate layer of the root. Here they grow firmly and form a docking station, so to speak. From this, the fungus expands outward and forms tree-like looking hyphae (chains of fungal cells, like small roots). This is also the origin of the name of the arbuscular mycorrhizal fungi, because arbus comes from Latin and means tree.

Figure 26: Arbuscular arm of the fungus growing into the cell lumen. (Remy et al. 1994)

These fungi improve nutrient uptake and help plants cope with biotic and abiotic stress by colonizing the root cells of most plants. This increases the root surface area and thus promotes both water and phosphorus uptake considerably in combination with the release of organic acids and phosphatases. Phosphorus uptake radius was increased from several mm to as much as 11cm (JAKOBSEN et al. 1992). This is statistically significant and essential for optimal yield in the living soil system.

Rhizophagus irregularis, formerly known as Glomus intraradices, and the very prominent Glomus mossae, are examples of PGPF strains that promote growth of a variety of crops, including soybean, hemp, and tomato. (Błaszkowski 1994; Bever et al. 2001)

Ascomycota

Ascomycota is a phylum that includes a wide variety of fungi, many of which have been shown to have PGP properties. An example of a PGPF from this phylum is Trichoderma spp. which can promote plant growth by producing enzymes that degrade complex organic molecules and release growth-promoting substances such as indoleacetic acid (auxin hormone). Trichoderma can also improve plant tolerance to environmental stresses and strengthen plant defense mechanisms against pathogens (ISR -> see Chapter 2.3.2.1 Indirect Mechanisms).

Trichoderma is also known to produce a variety of enzymes, including cellulases, chitinases, proteases, and cellulases (CWDEs = cell wall degrading enzymes), which can hydrolyze the cell walls of other fungi and give them a competitive advantage over other fungal pathogens. These enzymes can support the degradation of organic material and release nutrients for plant uptake.

It is also important to know that different Trichoderma species have different plant infection capabilities. Trichoderma virens and Trichoderma koningii, for example, are known pathogens and can damage plants, while Trichoderma harzianum and Trichoderma asperellum promote plant growth.

Basidiomycota

Basidiomycota is another phylum that includes a variety of fungi, including mushrooms. An example of a PGPF from this phylum is Laccaria bicolor, which can promote plant growth by improving nutrient uptake and increasing plant tolerance to environmental stress. This fungus can also produce enzymes that degrade complex organic molecules, and has been shown to increase growth and root biomass in a number of plants.

Those interested in more facts and culture-specific characteristics of the strains should definitely read the review (Hossain et al. 2017) and (Devi et al. 2020) read.

Nematodes

Entomopathogenic nematodes (EPNs), commonly referred to as beneficial nematodes, are a variety of roundworms that live in the soil. They are known to contribute to soil improvement, inhibit pathogens and enhance plant development.

Beneficial nematodes come in a variety of forms, each with its own special characteristics and modes of action. The two genera most commonly used in biological control are Steinernema and Heterorhabditis. These nematodes are known to infest and kill a wide variety of invertebrates and soil-dwelling insects, including termites, pinworms and grubs. They infect their host through natural openings such as the mouth, anus, or respiratory pores. Once inside, they release symbiotic bacteria for them that destroy the host.

These nematodes are known to improve soil health by reducing insect populations and increasing nutrient availability.

Plant pathogenic nematodes can be suppressed by some beneficial nematodes, including species of the genus Phasmarhabditis. Known plant-parasitic nematodes such as root knotweed and dagger stick nematode can be infected and killed by these worms. When controlling plant-parasitic nematodes in soil, they can be used as biological control agents.

Because there are so many different beneficial uses, nematodes can be characterized by how they operate.

Figure 27: Systematics nematodes (Abd-Elgawad et al. 2017)

Again, as with the last chapters: Read reviews and form your own opinion (Abd-Elgawad et al. 2017) Chapters 1-3 should be suitable for this.

Furthermore, there is an exact listing of suitable hosts for the nematode species mentioned above. However, it is important to note, especially if you do not have a completely closed system, that you only use "native" species. Firstly for the sustainability mindset that one should have at least halfway and secondly due to the invasive nature of some species listed here.

Figure 28: Suitable hosts (insects) for Steinernema and Heterorhabditis sp. (Abd-Elgawad et al. 2017)

Protozoa

Protozoa are a diverse group of protozoa that play a critical role in the soil food web. They can be divided into several groups, including amoebae, ciliates and flagellates, each with different characteristics and ecological functions.

In the soil, protozoa consume bacteria, fungi and other microorganisms, regulating their populations. This consumption in turn affects the availability of nutrients such as nitrates and phosphates to plants. In addition, protozoa can also release nutrients from organic material, making them more available to plants. For example, Amoeba spp. and Vorticella spp. are known to play an important role in decomposing organic material and releasing nutrients.
But hormonal feedback is also sent to the plant. Auxin, for example, in the form of IAA, which is responsible for apical growth (growth along the top) of the roots, is released, suggesting to the plant that it can continue to expand in that direction, given protozoa, i.e., nutrient supply.

Figure 29: "A conceptual model illustrating the hormonal effects of microfauna on root growth, modified from Bonkowski & Brandt (2002). Root excretions (1) stimulate the growth of a diverse bacterial community (Bonkowski 2004)

Protozoa also play a role in controlling plant pathogens. Some species, such as Rhizomonas spp. consume bacteria that can cause plant diseases, helping to protect plants from infection.

One example of specific species that play a critical role in the soil food web is the genus Tetrahymena. These are ciliates that have the ability to break down cellulose in plant material to make it available for plant uptake and promote plant growth. This ability makes Tetrahymena a valuable biofertilizer for sustainable agriculture.

Protozoa fall into several categories based on their characteristics and ecological role. Below are some of the major groups of protozoa, examples of species within each group, and examples of how they have been shown to affect the soil food web and/or plant growth:

• Amoebae: Amoebae are single-celled organisms that use pseudopodia to move around and play an important role in decomposing organic matter and releasing nutrients. Examples of amoebae that have been shown to affect the soil food web and/or plant growth include Acanthamoeba spp. and Dictyostelium discoideum. Acanthamoeba spp. for example, have been shown to consume bacteria and fungi, regulating their populations; they also release nutrients from organic material, making them more available for plant uptake. Dictyostelium discoideum has been shown to be a soil protozoan that promotes plant growth by releasing phosphorus from organic material.
• Ciliates: Ciliates are unicellular organisms characterized by the presence of cilia, which they use for locomotion. Examples of ciliates that have been shown to affect the soil food web and/or plant growth are Tetrahymena spp. and Rhizomonas spp. Tetrahymena spp. has been shown to degrade cellulose in plant material, making it available for plant uptake and thus promoting plant growth; it also plays a role in the decomposition of organic matter and the release of nitrogen and phosphorus. Rhizomonas spp. have been shown to consume bacteria that can cause plant diseases, helping to protect plants from infection.
• Flagellates: Flagellates are unicellular organisms characterized by the presence of flagella, which they use for locomotion. Examples of flagellates that have been shown to affect the soil food web and/or plant growth are Vorticella spp. and Colpoda spp. Vorticella spp. has been found to consume bacteria, regulating their populations, and has also been found to release nitrogen and phosphorus into the soil, making them more available for plant uptake. Colpoda spp. has been shown to play a role in nutrient cycling by breaking down organic matter and releasing nutrients.

Worms

Earthworms, which belong to the phylum Annelida, play a critical role in the soil food web by improving soil structure, aerating soil, increasing its water-holding capacity, and recycling nutrients. They are classified into three main groups: epigean, endogean, and anoecious earthworms, each with unique characteristics and ecological functions. (Palm et al. 2013)

Earthworms are known to consume large amounts of soil and organic matter, thereby mixing and aerating the soil and increasing its porosity and water-holding capacity. This improves soil structure and fertility, which in turn benefits plant growth. However, this is again dependent on the soil itself. Worm digestion improved the new polyvalent bonds, but also loosens the clay bonds in the soil to some degree. That is, the higher the clay content of the soil, the less significant the improvement in soil structure. (Schrader and Zhang 1997) However, in our peat this is irrelevant due to the absence of clay and it can be said that worm humus generally improves structure.

One of the earthworms' most important contributions to nutrient cycling is their digestive system, a complex process that involves the release of enzymes and microbes. The earthworm gut contains several enzymes that break down organic material, including cellulase, which breaks down cellulose, and protease, which breaks down proteins. The earthworm gut also contains a diverse community of microbes, including bacteria and fungi that further break down organic matter.

The decomposition of organic material by earthworms leads to the release of various nutrients such as nitrogen, phosphorus and potassium, which are chelated in the earthworm gut. Chelated nutrients are minerals that are bound to organic molecules so that they can be better absorbed by plants. This process occurs through the action of enzymes called chelating agents, which are produced by the earthworms or their gut microbes. These chelating agents include siderophores and phytosiderophores, which chelate iron, and organic acids such as citrate, gluconate and oxalate, which chelate other metals such as zinc and manganese.

Epigeic earthworms such as Eisenia fetida (redworm) live on the surface of the soil and mainly decompose organic matter. They are often used in composting systems, where they efficiently break down organic waste and improve soil fertility, which benefits plant growth.

Endogeic earthworms, such as Aporrectodea spp, are burrowing organisms that live in the topsoil and subsoil. They create horizontal tunnels and improve soil structure by mixing organic matter and mineral soil, which promotes water infiltration and aeration. They also contribute to nutrient cycling by breaking down organic material and releasing nutrients, especially phosphorus and nitrogen, which are important for plant growth.

Anecious earthworms such as Lumbricus terrestris create deep vertical burrows in the soil that improve soil structure by increasing porosity and water infiltration, and they also bring deeper nutrients closer to the surface. Their burrows also serve as drainage channels and improve soil oxygenation.

In indoor soil systems, epigeic and endogenous earthworms can be useful for breaking down organic matter and improving soil fertility, while anecic earthworms can improve soil structure and aeration. By using a combination of earthworm species, indoor soil systems can benefit from the diverse ecological niches and functions of different earthworm groups, which in turn can lead to better plant growth.

Figure 30: Involvement of worms in pedogenesis (soil formation). (Babu Ojha and Devkota 2014)

It should be noted that earthworm populations and diversity may vary depending on the indoor system and management practices used. It is also important to have optimal moisture, temperature, and light conditions for the earthworm to survive and thrive. Also, consider the effects of applying pesticides or other chemicals that could harm the earthworm population.

In addition to their positive effects on soil structure and fertility, earthworms can also play an important role in regulating soil microbial communities. Earthworms have been shown to selectively feed on certain types of microorganisms and promote the growth of beneficial bacteria that can improve soil health and plant growth.

More detailed information can be found on google scholar or specifically in the review of (Lavelle 1988) or (Edwards and Arancon 2004)

Arthropods (arthropods)

Arthropods are a diverse group of animals that include insects, spiders, crustaceans, and millipedes. They play an important role in the soil food web, serving as decomposers, predators, and prey. They have various ecological functions and can have significant impacts on soil health and plant growth, especially in indoor living soil systems.

Arthropods important to the Soil food web include several groups of insects, such as beetles, springtails, and mites. Within these groups, there are many different species that can have different effects on soil health and plant growth.

For example, beetles of the Scarabaeidae family, such as the common dung beetle (Scarabaeus sp.), play an important role in decomposing animal waste and contribute to nutrient cycling in the soil. These beetles burrow into manure and help bury and decompose it, improving soil structure and fertility. However, we do not want them in our indoor soil, as we also do not use uncomposted/already turned animal products. This would in fact provide a perfect breeding ground for Salmonella ssp. and other harmful organisms that do not harm the plant, but are toxic to humans. This would even lead to flunking the purity tests of the flowers. (Deutscher Apotheker-Verlag Doktor Roland Schmiedel 2021; Kim and Jiang 2010).

Another group, the springtails (Collembola), are tiny, wingless insects that are common in soil. They are efficient decomposers and predators of other soil organisms and can help control pests and diseases in the soil. For specific information on this species, I recommend the website, which was really built with enthusiasm and contains really nice pictures, like this one (A Chaos of Delight 2023).

Figure 31: White springtail (A Chaos of Delight 2023)

Mites, especially from the Oribatida family, are common in soil and play an important role in breaking down organic matter and controlling pest populations. They feed on dead plant material and microorganisms, degrade them, release nutrients and contribute to the build-up of organic matter, which can have a positive effect on plant growth and soil health.

Due to our indoor soil and changing conditions from warm/humid to more cool/dry throughout the life cycle of the plant, climate specific arthropod populations change.

There are several arthropod groups and species that specifically thrive under hot and humid conditions, including those found in peaty soils. For example, some springtail species (Collembola) are known to tolerate high temperatures and humidity and are found in peat bogs and other wetlands. Certain mite species (Oribatida) are also common in peat soils and can play an important role in the decomposition of organic matter.

Other arthropods found in tropical, moist environments include weevils (Staphylinidae), which can play a role in the soil food web as decomposers, predators, and prey, and are found in a variety of habitats, including peat soils.
Thus, these groups thrive better in vegetative, warm/humid, conditions.

On the other hand, certain beetle species such as ground beetles (Carabidae) can tolerate low humidity in colder and drier conditions and are found in arid and semi-arid regions. As predators, they play an important role in the soil food web and can also be useful in pest control.

In addition, certain spider species, such as wolf spiders (Lycosidae), can tolerate low humidity and are found in a variety of habitats, including dry desert regions.

These would be species that we can use towards the end of flowering. Since they cope with it very well than resistant. However, there are also species that work but cannot perform their optimal performance under these conditions. But you can still use or even prefer them to the optimal performers if they have specific prey schemes, are easier to handle or have less impact on the bloom.

Phytoseiulus persimilis, a spider mite predator that tolerates low humidity and cool temperatures. This species is known as an effective biological control agent against spider mites, which are widespread in many crops and can thrive in dry environments.

Another example is Amblyseius californicus, a predatory mite that is effective in low humidity and low temperatures. It is known to feed on various mite and thrips species and has proven effective in greenhouses, nurseries and outdoors.

A third example is Neoseiulus cucumeris, a predatory mite species that feeds on thrips and spider mites. It tolerates both low humidity and low temperatures and is often used as a biological control agent for thrips in greenhouses and outdoors.

It is important to note that many arthropod populations are habitat specific, meaning that certain species thrive in certain soils and habitats. It would be beneficial to study the local arthropod populations in the target habitat before introducing a species, as it may not be able to survive or adapt to the new conditions.

In addition, a distinction can be made between microarthropods and macroarthropods, these differ mainly in their influence on the overall soil structure. Macroarthropods are capable of restructuring soil profiles or displacing large amounts of soil, while microarthropods usually inhabit the pore space in the soil (and play little part in its modification). Ants, in particular, are popular macroarthropods because their burrows almost count them as ecosystem engineers. They are of immense importance outdoors but again in the indoor system too aggressive towards the seedlings at the beginning and in their way to keep aphids as farm animals.(Neher & Barbercheck, 2019) Species which are really useful for us are e.g. spiders like the wolf spider, which is used e.g. also in cranberries as pathogen hunter. Here, however, care should be taken either to choose spiders that occur in our area or to keep the system so closed that an escape is not possible.

Ridding the soil of heavy metals by microbes

With a combination of different microbes, impurities in the soil such as heavy metals can be bound and removed. Heavy metals lead to failing health tests in commercial cultivation and cause severe nerve damage when consumed. This must be prevented and therefore a combined approach is needed to bind the heavy metals. The most important representatives here are:

• Cadmium
• Chrome
• Mercury
• Nickel
• Lead
• Copper
• Zinc
• Arsenic

Cadmium is allowed here in the lowest amount and zinc in the highest, but they should all be avoided preventively or bound curatively for the soil in microbes. Most heavy metals are also toxic to the species mentioned here, but these have evolved sophisticated mechanisms for detoxification by polymerization in cell walls or degradation by enzymes. This also makes them inaccessible to plants such as our hemp, which is particularly high in heavy metals. Most can even use these degradation products for their growth afterwards. Thus Pleurotus ostreatus decomposes crude oil among other things into potassium. The most important organisms for this are:

• Trichoderma ssp. = lead, cadmium, and arsenic. (Govarthanan et al. 2019; Zhang et al. 2018).
• Bacillus and Pseudomonas aeruginosa = zinc and copper. (Rajendran et al. 2003)
• Zooglea spp. and Citrobacter spp. = copper, nickel, cadmium, cobalt.(Verma and Kuila 2019)
• Citrobacter spp. and Chlorella vulgaris = cadmium, lead, uranium, copper, nickel, mercury, uranium. (Rajendran et al. 2003)
• Aspergilus Niger = cadmium, thorium, uranium, zinc. (Rajendran et al. 2003)
• Pleurotus ostreatus = cadmium, copper and zinc. (Rajendran et al. 2003)
• Rhizopus arrhizus = cadmium, mercury, lead.(Rajendran et al. 2003)
• Stereum hirsutum = cadmium, copper, cobalt, nickel (Verma and Kuila 2019)
• Phormidium valderium = cadmium; lead (Verma and Kuila 2019)
• Ganoderma applantus = copper, lead, mercury. (Verma and Kuila 2019)

The best review I have found on the general topic of bioremediation by white rot fungi is: (Rajendran et al. 2003). There are also many other uses listed, such as conversion of crude oil / motor oil to plant nutrients and environmentally safe minerals.

The most important bioremediation processes are bioaccumulation, bioleaching, biosorption, biotransformation, and biomineralization, as well as metal-microbe interactions. These processes allow heavy metals to be rendered harmless (Verma and Kuila 2019). However, for this to happen, some species that store it in their fruiting bodies must have it removed, otherwise, if the fungus dies, the metals will return to the soil.

Microorganisms are useful not only for metal dissolution, but also for the reduction and oxidation of transition metals.

Figure 32: Mechanisms of metal tolerance in microbes. (Rajendran et al. 2003)

Pathogenic pests

This chapter will probably be the one that we will consider the shortest, compared to its size. Because there is own training to the pest control professional and to summarize it would be really utopian.

We therefore limit ourselves to the organisms with the highest damage potentials and their spread, prevention and "cure". We look at 3 examples each for the 3 groups bacteria, viruses/viroids and fungi. But for this we need short definitions of the most important terms in plant protection.

Bacteria

Xanthomonas campestris pv. cannabis

Bacterial leaf spot disease of hemp is characterized by necrotic lesions about 1-2 mm in diameter with a yellow halo 2-3 mm wide on the leaves. It invades the plant through wounds (Netsu et al. 2014). In some crops such as tomato, it also induces black rot of stems, but I have not yet found a source on this effect specifically in cannabis.

Transmission occurs through contaminated seed, water, and equipment. Preventive measures include using disease-free seed, sterilizing equipment and avoiding overhead irrigation.
While the bacteria do not penetrate the seed, they can colonize the seed surface and persist there until germination. There they infect the seedling at the base and destroy it.

Curative measures include the use of chemical control methods such as copper hydroxide, streptomycin or oxytetracycline. However, this is not recommended in the Living soil system, as any life will also be destroyed. However, for mother plants of essential genetics that have no backup, sometimes you simply must resort to such means to achieve salvage. It is best to isolate the plant and spray only with the aid of adequate personal protective equipment (PPE) and approved quantity (see Fertilizer/Plant Protection Regulation).

Figure 33; Symptoms of Xanthomonas campestris pv. cannabis (Netsu et al. 2014)

Pseudomonas cannabina

This bacterium causes leaf spot disease and stem rot in cannabis plants. It infects the leaves, causing them to turn yellow and brown. Transmission occurs through contaminated water and equipment and is host specific. (Bull et al. 2010)

Preventive measures include the use of disease-free seed and sterilization of equipment. Curative measures include the use of chemical control methods such as copper hydroxide, streptomycin or oxytetracycline.

Phytoplasma (PH)

This group of bacteria causes witches' broom disease in cannabis plants. It infects the buds of the plant and causes them to stunt and form small, deformed flowers. Transmission usually occurs through insect vectors.
Preventive measures include controlling vector populations, using disease-free seed, and removing and destroying infected plant material.

Curative measures include the use of chemical control methods such as tetracycline or streptomycin (Chaube et al. 2015).

Viruses/Viroids

Latent hop viroid (HLVd)

This virus belongs to the potyvirus genus and causes latent infections in cannabis plants. Compared to viruses, viroids do not have a protective protein coat and are present as naked RNA strands.
This means the viroid can be present in the plant's virome but show no active symptoms until an appropriate stress or epigenetic changes cause the infection to break out. Symptoms include stunting, leaf malformation or chlorosis, brittle stems, and yield reduction up to 50%.
Chlorosis and yield reduction are among the non-specific symptoms that occur with many viruses/diseases. I.e. often the viroid cannot be identified directly by observation. For this, Rt-qPCR analysis for viroid RNA particles is necessary to really know that it is this specific viroid.
It is transmitted by contaminated seeds, pollen, vectors (insects that ingest it and transmit it when feeding on plants) and mechanically (scissors, hands, etc).
Preventive measures include absolute cleanliness of equipment, reduction of the plant-feeding insect population, and quarantine/testing when new cultivars are added to the plant. (Warren et al. 2019; Flores et al. 2005; Chiginsky et al. 2021)

So far, only meristem culture in combination with multiple testing on multiple plant organs and at different stages of the plant's life has promised a cure.
Through molecular breeding using molecular markers, high throughput sequencing technologies and large selection/diversity populations, resistant cultivars can be found and bred into elite lines. This requires deep expertise and large amounts of investment, but it would be feasible and would pay off its investment hundreds of times over. My master thesis will deal with this very topic and the first research work is already in progress.

A new emerging startup in this field is the In Vitro / Biotechnology company AlpineBioLabs, which is currently being founded. This will cover all areas of virus/viroid prevention, treatment and research on this topic.

After infection of a living soil bed with HLVd it is unfortunately necessary to remove the soil. There are very few studies on this, but it can be said that HLVd can only survive permanently in living plant parts. However, one often works with companion plants, which can then also be infected and thus provide shelter for the viroid. More detailed studies are needed to determine how long the viroid can survive in dead roots. Therefore, it is essential to prevent infection in the first place, the basis is a mature prevention concept in connection with clean starting material. This requires either an own breeding with monthly tests and quarantine programs or a partner who provides fresh, clean mother plants every three months and keeps an extensive, secure gene bank with backups.

Again, advertising on our own behalf, AlpineBioLabs will face these challenges and try to become the trusted partner for virus/viroid prevention/treatment in the DACH/EU region.

Lettuce chlorosis virus (LCV)

This virus belongs to the genus Criniviruses and the family Closteroviridae

It causes interveinal chlorosis, brittleness, and occasionally necrosis in older leaves on cannabis plants. It is transmitted by the whitefly vector. General symptoms include yellowing and stunted growth of the plant.

There are no cures for viral infections, so preventive measures such as the use of disease-free seed and equipment and control of the vector, the whitefly, are critical to controlling the disease.

As with almost all viruses/viroids, a meristem culture with integrated test protocol is the solution. Here, tissue that is not connected to the guidance channels (xylem/phloem) can be excised. This tissue (meristem) acts like stem cells and from them, any plant organ can be differentiated using organogenesis.

Figure 34: "Cannabis salatchlorosis virus (LCV) transmitted via shoots.(a) Stunted growth of a cannabis plant propagated from LCV-infected shoots (left) compared to growth of a cannabis plant propagated from uninfected shoots(right). (Hadad et al. 2019)

Cannabis cryptic virus (CanCV)

Cannabis Cryptic Virus (CanCV) is a virus that causes the symptoms of "hemp streak disease" in cannabis plants. The virus, which is currently the only known virus of the genus Caulimovirus infecting cannabis, was first identified in Italy in 2015.

Symptoms of CanCV infection include yellow or brown streaks on leaves, stunted growth and yield reduction. The virus is transmitted through the use of infected planting material and through contact with infected plant parts.

One of the most important effects of CanCV on horticulture is that it can significantly affect the yield and quality of cannabis plants. The virus can cause stunted growth, resulting in smaller plants with fewer flowers and lower THC and CBD levels.

Another impact of CanCV is that it can be difficult to control. So far, no studies on mechanical (horizontal) transmission are available. That is, so far it is thought to be transmitted exclusively vertically, i.e., via gametes (pollen/flowers) and thus seeds (Righetti et al. 2018)

Currently, there are no known chemical treatments for CanCV, and the most effective control measures involve the use of sanitation and virus-free planting material.

Again, meristem culture is the only curative solution. (Ziegler et al. 2012)

Fungi

Botrytis cinerea (BC)

This fungus causes gray mold on cannabis plants. It attacks the flowers, leaves and stems of the plant and causes wilting and death. It is transmitted by spores that are spread by wind or water. Preventive measures include using disease-free seeds, avoiding overhead irrigation, and removing and destroying infected plant material.

This fungus can infect both the live plant and drying flowers. Most will have already made acquaintance with it. The most important thing to avoid infection, besides preventing transmission, are the environmental conditions. In particular, free water, which occurs through condensation, provides the best breeding ground for Botrytis. It cannot survive on humidity alone. I.e. either plant sap has leaked out due to damage to the tissue (acute Ca2+ deficiency + mechanical action) or condensation is present.

The complete loss of medical cannabis in Israel due to B. cinerea is estimated to be 10% of total production in a year (Jerushalmi et al. 2020)

Alternaria alternata

Alternaria alternata is a fungal pathogen that can affect a variety of plant species, including cannabis. The fungus causes a disease called Alternaria leaf spot disease, which is characterized by the development of dark brown or black lesions on the leaves, stems and flowers of the plant.

The fungus infects the plant through natural openings such as stomata or wounds, but it can also enter through the seeds. Once the fungus has infected the plant, it begins to produce spores that are spread by wind and water, allowing the fungus to infect new plants.

Symptoms of Alternaria leaf spot disease include the formation of dark brown or black lesions on the leaves, stems, and flowers of the plant. These lesions can vary in size and shape and result in leaf yellowing, defoliation and death of the plant. The fungus can also infect buds and flowers, dramatically reducing yield and crop quality.

"In August 2000, for example, an outbreak of leaf spot disease in broadleaf tobacco (Nicotiana tabacum L.) caused by A. alternata resulted in the loss of 75% and 89% of total acreage in Connecticut and Massachusetts, respectively." (LaMondia 2001)

The fungus can survive on plant debris and survive in the soil for long periods of time. It is known that the fungus can infect plants at high relative humidity, so the disease is more pronounced in humid environments.

Figure 34: (A) Alternaria alternata (isolate 22A); (B) Botrytis cinerea (isolate 63A); and (C) control. (Jerushalmi et al. 2020)

Fusarium oxysporum/solani

Fusarium oxysporum and Fusarium solani are two different species of the fungus Fusarium known to cause plant diseases. While both species can infect a variety of plant hosts and cause similar symptoms e.g. cannabis, there are some differences in the symptoms they cause.

Fusarium oxysporum is known to cause a disease called Fusarium wilt, which is characterized by wilting and yellowing of the leaves and subsequent death of the plant. This pathogen infects the plant through the root system and colonizes the stem, causing wilting and death of the plant. Symptoms may also include leaf chlorosis (yellowing) and reduction in size of leaves, flowers and fruits.

Fusarium solani, on the other hand, is known to cause a disease called Fusarium crown rot, which is characterized by wilting and yellowing of the leaves and subsequent death of the plant. This pathogen infects the plant through the root system and colonizes the stem, causing wilting and death of the plant. Symptoms may also include leaf chlorosis, leaf necrosis (browning), and a reduction in the size of leaves, flowers, and fruits. To control these pathogens, it is important to use effective disease control strategies. These include regular monitoring of plants for symptoms of fungal infection, implementation of preventive measures such as crop rotation, sanitation, and the use of disease-free planting material, and the use of fungicides under the guidance of a plant pathologist or other qualified professional in extreme emergencies. (Ali Merjan 2017)

It is important to note that further research is needed to identify new and effective strategies to control these and other fungal pathogens that infect medical cannabis. In addition, research into the genetic and molecular characteristics of these pathogens could help identify new ways to control them. (Jerushalmi et al. 2020)

Figure 35: (A) Nutrient saline images of intact plants, including roots of (C) a plant inoculated with nutrient saline (control) compared to a plant inoculated with Fusarium oxysporum (isolate 64B) (D). (Jerushalmi et al. 2020)

These were now only pathogens (except botrytis) that affect living plants. But there are hundreds of others like Asperagillus ssp. etc. that can contaminate flowers and also have to be tested for according to the DAB.

Endophytes

"Understanding Cultivar Specificity and Soil Determinants of the Cannabis Microbiome." (Winston et al. 2014) and "Cannabis Microbiome and the Role of Endophytes in Modulating the Production of Secondary Metabolites: An Overview" (Taghinasab and Jabaji 2020) are two recent studies that examine the relationship between the endophytic microbiome of cannabis plants and their growth, as well as the effects of endophytes on the production of secondary metabolites in cannabis. Endophytes are microorganisms that live in the tissues of plants and can have various beneficial effects on their host, such as increased resistance to pathogens, enhanced nutrient uptake, and modulation of secondary metabolite production.

The first study, titled "Understanding Cultivar-Specificity and Soil Determinants of the Cannabis Microbiome," focused on two main factors that might influence the endophytic microbiome of cannabis plants: Cultivar Specificity and Soil Determinants. The researchers collected samples of cannabis plants grown in different soils and cultivars and used DNA sequencing techniques to identify and quantify the different endophytic microorganisms present.

The results showed that the endophytic microbiome of cannabis plants was indeed influenced by both cultivar specificity and soil factors. Different cannabis cultivars had different endophytic microbiomes, with some cultivars harboring a greater diversity of microorganisms than others. In addition, the endophytic microbiomes of cannabis plants grown in different soils were also different, with some soils supporting a greater diversity of endophytes than others. In particular, soils with higher organic matter content supported greater diversity of endophytic microorganisms, especially Proteobacteria, Actinobacteria, and Bacteroidetes. These endophytes have been shown to promote plant growth by producing plant growth-promoting compounds such as cytokinins and indoleacetic acid. In addition, soils with neutral pH (around 7) have been found to have higher levels of endophytes belonging to Phyla Firmicutes and Acidobacteria, which have been shown to enhance plant nutrient uptake.

The second study, "Cannabis Microbiome and the Role of Endophytes in Modulating the Production of Secondary Metabolites: A Review," focuses on the effects of endophytes on secondary metabolite production in cannabis. The study found that endophytic microorganisms can modulate the production of secondary metabolites in cannabis by affecting the expression of genes involved in the biosynthesis of these compounds. Specifically, endophytes belonging to the genus Burkholderia were found to positively correlate with the production of secondary metabolites in cannabis such as terpenoids and flavonoids. These endophytes have been shown to produce compounds such as siderophores and enzymes that can enhance the uptake of iron and other essential nutrients, which in turn could lead to an increase in the production of secondary metabolites.

Another hypothesis is the influence of hormone production by endophytes. The gibberellin produced positively influences the synthesis of THC/CBD (terpenoid-based substances) by regulating 1-aminocyclopropane-1-carboxylic acid levels, leading to an increase in ethylene levels. (Mansouri et al. 2011)

Figure 36: "The most common endophytes found in different tissues of Cannabis sativa plants from different geographic regions." (Robinson et al. 2016)

Outlook

Now this material should be enough to keep you busy for a few weeks, it took me several years of steady learning. You don't have to know everything or understand everything, but the better you understand your tools as a grower, the better your results will be.

In the next part, we will look at how to put this theoretical knowledge into practice in order to create your own soil mix. It will be about a deep analysis of the different substrates, aeration materials, compost forms, ferments, additives like Biochar and many more amendments. It is also filled with reference values to calculate the subsequent soil properties.

The final part will take place during the summer after the first field tests with the soil mix. Here the running conditions will be simulated and compared with soil tests. A test overview and calculation examples with correct field values will also be covered here, in order to finally arrive at a final recipe, which you can then reproduce yourself.

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by Max